The rats were then killed by exsanguination from the inferior vena cava and the major organs, including the lung, chest wall, brain, liver, kidney, spleen and mediastinal lymph nodes, were fixed in 4% paraformaldehyde and processed for histological examination.Analysis of inflammatory reaction in the pleural cavityCells in the lavage fluid were counted using a hemocytometer (Erma Co., Ltd, Toky