In further desired embodiments of the present invention, X and Y are O; and R1 and R2 are independently H or OH; B is a nucleoside base and L is a label which may be a chromogenic, fluorogenic or a chemiluminescent molecule. [0045] In one embodiment of the method of detecting an analyte provided herein, the steps include anchoring a nucleic acid template to the analyte, conducting a DNA polymerase