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  • Organic solvents, pH value, concentration of the buffer, and temperature have significant effects on enzyme activity but have little effect on enzyme selectivity.Preferably, 5%-50% organic solvents, including acetonitrile, dimethyl sulfoxide (DMSO), tetrahydrofuran, acetone, dimethyl formamide (DMF), dioxane, isopropanol, ethanol, methanol, or the like, should be present in the reaction mixture to
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