The liposomes may be prepared by mixing in a flask of suitable size desired amounts of lecithin, sterol with or without a charged component of phosphatidic acid, dicetyl phosphate or stearylamine in an organic solvent, such as chloroform, dichloromethane diethyl ether, carbon tetraoxide, ethyl acetate, dioxane, cyclohexane, and the like (chloroform being preferred).