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Long term chloroform: isoamyl alcohol treatment ensured removal of chlorophyll and other colouring substances such as pigments, dyes etc. that hinder the quality of DNA. The popular CTAB method (Doyle & Doyle, 1990) which focused on the removal of polyphenolics and/or polysaccharides, often neglected the importance of eliminating RNA. Many DNA isolation procedures also yielded large amounts of RNA
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