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For repeated application of the present biosensor, the pyranose-oxidizing enzyme, and the polysaccharide hydrolase, if necessary, can be immobilized on the hydrophilic polymer layer through crosslinking with glutaraldehyde or immobilized on the hydrophilic polymer layer together with a polymeric material such as nitrocellulose, cellulose acetate, and polyacrylonitrile.
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