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For immunohistochemistry, the tissue sample may be fresh or frozen or may be embedded, for example, in paraffin or other waxes, nitrocellulose, carbowax (also known as water soluble polyethylene glycol (see, Gao ed. (1993) ???Polyethylene Glycol as an Embedment for Microscopy and Histochemistry,??? CRC Press, Inc.
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