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wherein B comprises a nucleoside or nucleotide base, such as uracil, cytosine, deazaadenine, or deazaguanosine; W1 and W2 taken separately can be ???OH or a group capable of blocking polymerase-mediated template-directed polymerization, e.g., ???H, fluorine, and the like; W3 can be OH, or mono-, di- or triphosphate or a phosphate analog; and D is a dye compound of the present teachings.
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