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<http://webisa.webdatacommons.org/172995373>	<http://www.w3.org/ns/prov#value>	"Other dissociating agents such as urea, and alcohols such as isopropanol or methanol should be substitutable for GuHCl and EtOH. Following the initial dialysis, the protein was dialyzed against the load buffer for the final HPLC purification step." .