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  • Total RNA or poly (A)+ RNA isolated from the target source is reverse transcribed to make double stranded cDNA. The primer used for the first strand cDNA synthesis is oligo (dT)12-24, oligo (dT)12-24V, or oligo(dT)12-24VN with or without anchor primer at the 5???-end, wherein V is any nucleotide other than T such as A, G, or C, and N is any nucleotide A, T, G, or C. The presence of a degenerate nu
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