However, one criticism in particular is fundamental to determining the likely power and accuracy of any final database used for species identification???the effect of sampling (Moritz and Cicero 2004; Meyer and Paulay 2005; Meier et al. 2006; Wiemers and Fiedler 2007; Zhang et al. 2010; Hendrich et al. 2010; Virgilio et al. 2010).