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  • SPLV were prepared by the lipid hydration performed in an organic solvent, such as diethyl ether, according to Biochemistry, 24:2833-2842 (1985). 0.5 mM desferal was included in all aqueous solutions used for liposome preparation to minimize the effect of Fe ions or doxorubicin degradation and lipid peroxidation according to procedure described in U.S. Pat. No. 4,797,285 incorporated herein by ref
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