n is a C to T transition at nucleotide 34 (3rd position of the anticodon)spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.glnX44(AS) was formerly called supE44glnX44(AS) was formerly called su+IIglnX44(AS) was formerly called glnV44thiE1 was formerly called thi-1AS = amber(UAG) suppressorReferences: Beckwith, J.R., E.R. Signer 1966.