It has been demonstrated that IPA (isopropyl alcohol) is a useful reagent in the precipitation of N-acetylglucosamine and in the removal of sample darkening at 105?? C. However, it is very likely that a skilled person can choose other organic solvents that are miscible with water such as acetonitrile or ethanol to replace IPA and achieve the same effect.Preheat Followed by Water/IPA PrecipitationA