* Lyophilization ??? ??? ??? Desiccation dehydration of vegetative cells when directly exposed to normal room air ??? ??? ??? Lyophilization a combination of freezing and drying; used to preserve microorganisms and other cells in a viable state for many years ??? ??? ??? Are microbes adeversley affected by cold temps Some are but most are not, some freezing temps actually preserve cultures. ??? ??