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  • rs). (E???G) The pre-growth/pad transfer experiment can test the DILUTION mechanism. (E) Cells with an IPTG-inducible spo0A construct (Phyp-spo0A), but lacking the endogenous spo0A gene, are first grown for multiple generations on resuspension media pads without IPTG, diluting out nutrients or other factors present only in rich media.
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