Note the punctate nuclear p-H2AX in mutant myeloid cells (compare panels J and N with B and F). (Q) Western analysis of p-H2AX and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) loading control from emi1m/m or emi1wt/(wt or m) embryo lysates. (R and S) Whole-mount immunohistochemical analysis of p-H2AX in emi1wt/(wt or m) (R) and emi1m/m (S) siblings.