Total DNA from human serum (200 ??l) and urine (200 ??l) was prepared using QIAamp DNA Mini Kits (QIAGEN, Australia) according to the manufacturer's instructions.Table 1List of bacterial strains used for TaqMan PCRCollection and testing of human seraSingle serum samples were from 66 patients with clinically suspected leptospirosis or from at risk occupations or activities, for example persons in