Similarly, c-Jun and CREB were detected after immunoprecipitation with anti-ATF-2, suggesting that the interaction of CREB, ATF-2, and c-Jun was stimulation-dependent.Figure 5CREB, ATF-2 and c-Jun form a complex in response to T cell activationSince PBMC contain cells other than T-cells, we performed co-immunoprecipitation to study the interaction of these transcription factors in protein extracts