Light intensity, temperature, nutrients and pH are the most important factors for optimising the microalgae culture (Ogbonna and Tanaka, 1996; Sevrin-Reyssac, 1998; Zonneveld, 1998; Martinez et al. 1999; Zhang et al. 1999; John and Flynn, 2000; Carlozzi and Sacchi, 2001; Zhang and Lee, 2001; Babel et al. 2002; Kayombo et al. 2002; Yuan et al. 2002; Kayombo et al. 2003; Fiedler et al. 2003; Tukaj e