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  • In one preferred embodiment of the invention, the tracer is a fluorescent compound with low steric hindrance, such as fluoresceine, hexachlorofluoresceine (HEX), dansyl (edans), rhodamine, tetramethylrhodamine (5- or 6-TAMRA), carboxy-X-rhodamine (ROX), chromophores of the NIR type (LI-COR Inc, Lincoln Nebr., USA), cyanine derivatives such as Cy5 and Cy3 (Randolph J. B. et al., Nucleic Acids Res.,
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