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  • In addition, Prodigal implements a few more minor tweaks to the coding score, including boosting the score of particularly long genes (dependent on the GC content of the organism: ~700 bp or so in low GC, ~1200 or so in high GC) to be minimally positive if the preliminary coding score is negative.Once Prodigal has calculated coding potential scores for every start-stop pair in the genome, the next
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