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  • On the other hand, non-native states with spectroscopic and hydrodynamic properties intermediate between those of the fully native and unfolded states (the so-called molten-globule state) often accumulate under mildly denaturing conditions, such as acidic or basic pH.4???6 In some cases, deviations from simple two-state behavior can also be observed in denaturant-induced or thermal unfolding equil
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