A nucleic acid detection kit comprising: a) at least one terminal-phosphate-labeled nucleoside polyphosphate according to the formula: wherein P=phosphate (PO3) and derivatives thereof; n is 3 or greater; Y is an oxygen or sulfur atom; B is a nitrogen-containing heterocyclic base; S is an acyclic moiety, carbocyclic moiety or sugar moiety; P-L is a phosphorylated label, wherein L is a label contai