In one embodiment, the lysis buffer lyses blood cells or other cells or microorganisms. [00134] In one embodiment, the second chamber 103 of the separator/concentrator device contains a volume of diluting fluid, e.g., water or washing solution for rinsing the particulate matter, e. g. the second chamber can comprise at least a volume of about 1000 microliters or less than lOOO??L, for example, whe