FIG. 9A illustrates an activated surface, here exemplified as an epoxy surface of the type in FIG. 3A, interfacing with a protein which, prior to the imaging step, has been mixed with iminodiacetic acid and metal ion such as copper to form a metal coordination complex between the imidazole and IDA groups, shown in the center of FIG. 9A. Flanking the central histidine residue having the imidazole s