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  • Briefly, HeLa cell lysate (from approximately 30 L cells) was quickly thawed at 37 ??C supplemented in a mixture including 10% glycerin, 1 mM EDTA, 1 mM EGTA, 50 mM NaF, 50 mM beta-glycerol phosphate, 10 mM benzamidine, 1 mM DTT, and 1?? protease inhibitor mixture (Roche, Basel, Switzerland).
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