The concentration of the oligonucleotide is preferably between about 0.1 ??M to 10 ??M, more preferably between about 0.1 ??M to 5 ??M, and most preferably between about 0.5 ??M to 1 ??M.Also according to any of the above methods, the sample formed preferably includes salts such as NaCl and Mg2+.Also according to any of the above methods, at least a portion of the multiple cycles of primer extensi