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  • The real-time PCR reaction was performed as previously described [33].Quantitative analysis of IL-1??, IL-6, IL-8, IL-10, IL-12, TNF-?? and IFN-??The concentration of cytokines, including interleukin (IL-6), IL-8, IL-10 and IL-12p70, tumor necrosis (TNF)-?? and IL-1?? in culture supernatant with equal cell number loading, were measured simultaneously using human inflammatory cytokine Cytometric Be
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