The final set of proteins used for the analysis was composed of 109 sequences, with representatives of each PRDM member in all 7 species except for PRDM3 (missing in fugu), PRDM17 (missing in fugu and chicken), and the non-primate PRDM7/9 (missing in chicken and frog).For each PR domain of the different PRDM orthologous groups, we built a multiple sequence alignment (MSA) using MUSCLE [40], with t