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  • The conjugate used was a conjugate of the fluorescent dye Cy3 with a specific anti-digoxigenin antibody commercially available From Laboratoria Medigen, Novosibirsk, Russia. [0350] A solution of anti-digoxigenin antibody-Cy3 conjugate at an approximate concentration of 2 micrograms/ml was diluted 1:125in the blocking buffer.
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