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  • Fusing E. coli tet repressor with VP16 creates a tetracycline-controlled transactivator that in the presence of tetracycline or derivatives, can induce expression from polynucleotides operably linked to tet operators (Gossen et al., 1995); this approach was an improvement on an earlier one that lacked the VP16 fusion, wherein tetracycline is chronically administered to silence expression; removal
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