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  • antigen is an enzyme which catalyzes cleavage of the oligoi have done the indirect elisa by first coating the elisa plates with antibody and then capturing the antigenthen either oligo or antibody is incubated with it and then vice versa to check for the difference in the O.Dand in case of only one mab out of nearly 80 clones i could see a difference in the O.D indicating that the antibody is not
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