Agents such as urea and formamide, ...PCR reaction components... efficiency or specificity of PCR: Betaine (0.52 M) Bovine serum albumin (BSA; 100 ng/50 l) detergents Dimethylsulfoxide (DMSO; 210%) (v/v) Gelatine Glycerol (15%) (v/v) Pyrophosphatase (0.0010.1 ...The use of TaqMaster PCR Enhancer... as described (2).