doi:10.1371/journal.pone.0002428.g006To test whether CPA resistance is mediated by ALDH1 enzyme activity, UM-C4 or UM-C6 colorectal tumor cell colonies established in vitro over the course of 3???4 days were then exposed to the bioactive CPA-metabolite, 4-HC, for 4 hours without or including various concentrations of the ALDH1-specific inhibitor, DEAB. In the absence of DEAB, the EC50 of 4-HC was