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  • A minimization of contamination from nucleic acid sequences other than the known target sequence is desired for amplification in the present invention. [0064] The terminal-phosphate-labeled nucleotide useful in the methods and kits of the present invention may be represented by Formula I: [0065] wherein P is phosphate (PO3) and derivatives thereof, n is 2 or greater; Y is an oxygen or sulfur atom;
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