Figure 7 shows that the major GPEtn peak encompassing molecular species in the mass range m/z 724???732 (e-36:3 ??? a-36:0, Table S1) was considerably reduced in the TbECT cKO grown in the absence of tetracycline for 36 h, compared to either the parental cells or the TbECT cKO grown in the presence of tetracycline (Fig. 7, compare A and B with C).