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  • A tracking dye may be added to the protein solution to allow the experimentor to track the progress of the protein solution through the gel during the electrophoretic run.Besides the addition of SDS, proteins may optionally be boiled in the presence of a reducing agent, such as dithiothreitol (DTT) or 2-mercaptoethanol, which further denatures the proteins by reducing disulfide linkages, thus
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